Monodeiodination of Thyroxine to 3, 3′, 5-Triiodothyronine and 3, 3′, 5′-Triiodothyronine in Human Kidney Homogenate
نویسندگان
چکیده
منابع مشابه
Monodeiodination of thyroxine to 3, 5, 3'-triiodothyronine and to 3, 3', 5'-triiodothyronine in isolated dog renal cortical tubuli.
Monodeiodination of T4 to T3 and rT3 in the intact cells of dog renal tubuli and glomeruli was investigated. The tubuli and glomeruli were obtained by a sieve method. T4 (2 micrograms/ml) was incubated in Tris-HCl buffer, pH 7.5, with renal cells (180 micrograms protein/ml) and 5 mM DTT for 1 h at 37 degrees C and the T3 and rT3 generated during incubation were measured by specific radioimmunoa...
متن کاملCirculating 3 , 3 ' , 5 ' - Triiodothyronine ( Reverse T 3 ) in the Human Newborn INDER
samples was significantly higher than the level (41±2 ng per 100 ml) in 27 normal adult sera; the corresponding mean serum T4 of 12.7±0.8 ug per 100 ml in cord blood also was significantly higher than that (8.6±41.9 big per 100 ml) in 108 normal adults. By contrast, the mean serum T3 concentration in 15 cord blood samples, 24±3 ng per 100 ml, was significantly, lower than the value of 126±43.2 ...
متن کاملRadioimmunoelectrophoretic analysis of proteins binding 3, 3', 5'-triiodothyronine in human serum.
The binding of purified 131I-3, 3', 5'-triiodothyronine (reverse T3) (rT3) to normal human serum components was investigated by a radioimmunoelectrophoretic technique. When anti-whole human serum was used, five distinct arcs of radioactivity were observed. Evidence was obtained that five of these radioactive arcs were not artifacts, but were due to components binding rT3. From the radioimmunoel...
متن کاملEffects of butyl-4-hydroxy-3, 5-diiodobenzoate on the metabolism of I131-thyroxine and triiodothyronine.
Comparatively little is known about the interference in actions and metabolism of hormones by their appropriate analogues, though thyroxine has been studied in this regard more than have other hormones. One analogue which has been demonstrated by Maclagan et ~2. (1) to prevent the calorigenic effect of thyroxine is butyl+Ghydroxy-3,5-diiodobenxoate (BHDB). The mechanism of antagonism has not be...
متن کاملAn assessment of daily production and significance of thyroidal secretion of 3, 3', 5'-triiodothyronine (reverse T3) in man.
While 3, 3', 5'-triiodothyronine (reverse T3, rT3) has been detected both in human serum and in thyroglobulin, no quantitative assessment of its metabolic clearance rate (MCR), production rate (PR), or secretion by the thyroid is yet available. This study examines this information in euthyroid subjects and evaluates it in light of similar information about two other iodothyronines in the thyroi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Folia Endocrinologica Japonica
سال: 1982
ISSN: 0029-0661
DOI: 10.1507/endocrine1927.58.3_199